Hashem, Omar Adnan (2026) Development of a Spectrophotometric Method Based on a Schiff Base for the Determination of Sulfasalazine in Bulk Drug Substance and Pharmaceutical Dosage Forms. Central Asian Journal of Medical and Natural Science, 7 (4). ISSN 2660-4159

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Abstract

A simple, accurate, and sensitive spectrophotometric method was established for the quantitative determination of Sulfasalazine (SSZ) based on Schiff base formation. The method involves the condensation reaction between Sulfasalazine and terephthalaldehyde in an acidic medium, producing a stable, water-soluble yellow-colored product. The resulting Schiff base exhibits a maximum absorption peak at      (480 nm) and A UV–visible spectrophotometer was used to carry out the spectral measurements. The results showed that absorbance increased with increasing sulphasalazine concentration within the range (3–120 µg mL⁻¹), with a linear relationship in accordance with Beer–Lambert’s law. The molar absorptivity was recorded as 5,982.73 L/mol⁻¹ cm⁻¹, whilst the Sandel sensitivity was 0.0665 µg/cm⁻². The limit of detection (LOD) was 0.0592 µg mL⁻¹, whilst the limit of quantification (LOQ) was 0.1974 micrograms/ml. When the method was applied to commercial sulphasalazine tablets, it yielded accurate results, indicating its suitability for the quantitative analysis of pharmaceutical preparations.

Item Type:	Article
Uncontrolled Keywords:	Sulfasalazine, Terephthalaldehyde, Spectrophotometric, Schiff s bases, Beer’s law
Subjects:	H Social Sciences
Depositing User:	admin eprints
Date Deposited:	01 Aug 2026 09:45
Last Modified:	01 Aug 2026 09:45
URI:	http://eprints.umsida.ac.id/id/eprint/16893

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