Salman, Suhad Abdul Hussein (2026) GENOMIC ANALYSIS OF ANTIBIOTIC RESISTANCE GENES IN GUT MICROBIOTA BEFORE AND AFTER FECAL MICROBIOTA TRANSPLANTATION. Journal of Medical Genetics and Clinical Biology, 3 (7). ISSN 3032-1085

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Abstract

Objective: Fecal microbiota transplantation, or FMT for short (Figure 1), is a novel preventive and therapeutic strategy aimed at repopulating gut registers you want to use when treating recurrent infections, particularly in patients suffering from dysbiosis. Nevertheless, the impact was minor and inspired only limited engagement in queries concerning how the situation of antibiotic resistance genes (ARGs) inside the gut microbiome might be adjusted. Method: Using high-throughput metagenomic sequencing, this study profiled the gut microbiota associated with ARGs before and after FMT. Recipient stool samples were obtained pre-FMT and at multiple time points post engraftment. We performed bioinformatic analysis for identification and quantification of these ARGs, to assess shifts in the resistome as well as microbial diversity. Results: Results showed that the overall abundance and diversity of antibiotic resistance genes (ARGs) were decreased after fecal microbiota transplantation (FMT), microbial diversity was increased, and commensal bacterial populations were restored. Genes associated (highly enriched) with multidrug resistance and beta-lactam resistance exhibited pronounced decreases after transplantation. Comparison showed that the composition of donor microbiota was responsible for dominant effects on post-FMT resistome profiles in populations, indicating a potential role for selective donation to modulate ARG transmission and reduction. Further, network analysis suggested that FMT destabilized ARG-host associations, indicative of gut microbiome ecological disturbance. Novelty: Our findings demonstrate the potential of FMT not only to restore microbial levels but also to reduce the host burden of gut-resident antibiotic resistance. However, this study highlights the importance of bolstering safety and therapeutic outcomes through genomic surveillance to FMT protocols. This conclusion is limited by the need for more studies around ARG reduction durability and standardization approaches to base recommendations for donor screening and resistome monitoring.

Item Type:	Article
Uncontrolled Keywords:	Fecal microbiota transplantation, Antibiotic resistance genes, Gut microbiota, Metagenomic analysis, Resistome
Subjects:	H Social Sciences
Depositing User:	admin eprints
Date Deposited:	14 Aug 2026 23:59
Last Modified:	14 Aug 2026 23:59
URI:	http://eprints.umsida.ac.id/id/eprint/16967

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